Nano-Chemical Infrared Imaging of Membrane Proteins in Lipid Bilayers
|Reviews and Highlights||Quantum Science||Molecular and Soft-matter||Ultrafast Nano-optics and Nanophotonics||Mineralogy and Geochemistry|
The spectroscopic characterization of bio-molecular structures requires nanometer spatial resolution and chemical specificity. We perform full spatio-spectral imaging of dried purple membrane patches purified from Halobacterium salinarum with infrared vibrational scattering-type scanning near-field optical microscopy (s-SNOM). Using near-field spectral phase contrast based on the Amide I resonance of the protein backbone, we identify the protein distribution with 20 nm spatial resolution and few-protein sensitivity. This demonstrates the general applicability of s-SNOM vibrational nano-spectroscopy, with potential extension to a wide range of biomolecular systems.
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